Description: Rabbit polyclonal antibody to ETS1 (Phospho-S251) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S251 of human ETS1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ETS1 Alternative Names: EWSR2; Protein C-ets-1; p54Entrez Gene (Human): 2113Entrez Gene (Mouse) : 23871Entrez Gene (Rat) : 24356SwissProt (Human): P14921SwissProt (Mouse) : P27577SwissProt (Rat) : P41156Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ETS1 (Phospho-S251) expression in Hela (A), NIH3T3 (B), H9C2 (C) whole cell lysates.
-
Immunohistochemical analysis of ETS1 (Phospho-S251) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.