Description: Rabbit polyclonal antibody to NACA1 (Phospho-S43) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S43 of human NACA1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NACA Alternative Names: Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2Entrez Gene (Human): 4666Entrez Gene (Mouse) : 17938SwissProt (Human): Q13765SwissProt (Mouse) : Q60817Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NACA1 (Phospho-S43) expression in HEK293T (A), Jurkat (B), NIH3T3 (C), CT26 (D), H9C2 (E), PC12 (F) whole cell lysates.
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  Immunohistochemical analysis of NACA1 (Phospho-S43) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.