Description: Rabbit polyclonal antibody to DAP Kinase 1 (Phospho-S736) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S736 of human DAP Kinase 1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DAPK1 Alternative Names: DAPK; Death-associated protein kinase 1; DAP kinase 1Entrez Gene (Human): 1612Entrez Gene (Mouse) : 69635SwissProt (Human): P53355SwissProt (Mouse) : Q80YE7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DAP Kinase 1 (Phospho-S736) expression in HEK293T (A), CT26 (B), NIH3T3 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of DAP Kinase 1 (Phospho-S736) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.