Description: Rabbit polyclonal antibody to SMAD4 (Phospho-T276) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T276 of human SMAD4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SMAD4 Alternative Names: DPC4; MADH4; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4Entrez Gene (Human): 4089Entrez Gene (Mouse) : 17128Entrez Gene (Rat) : 50554SwissProt (Human): Q13485SwissProt (Mouse) : P97471SwissProt (Rat) : O70437Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of SMAD4 (Phospho-T276) expression in HeLa (A), HepG2 (B), mouse lung (C) whole cell lysates.
• 
  Immunohistochemical analysis of SMAD4 (Phospho-T276) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 
  Direct ELISA antibody dose-response curve using Anti-SMAD4 (Phospho-T276) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.