Description: Rabbit polyclonal antibody to CD45 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD45. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTPRC Alternative Names: CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45Entrez Gene (Human): 5788Entrez Gene (Rat) : 24699SwissProt (Human): P08575SwissProt (Mouse) : P06800SwissProt (Rat) : P04157Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD45 expression in HEK293T (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates.
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Immunohistochemical analysis of CD45 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.