Description: Rabbit polyclonal antibody to PKC alpha (Phospho-S657) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S657 of human PKC alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PRKCA Alternative Names: PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alphaEntrez Gene (Human): 5578Entrez Gene (Mouse) : 18750SwissProt (Human): P17252SwissProt (Mouse) : P20444SwissProt (Rat) : P05696Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PKC alpha (Phospho-S657) expression in HeLa (A), HEK293T (B), C6 (C), CT26 (D) whole cell lysates.
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Immunohistochemical analysis of PKC alpha (Phospho-S657) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.