Description: Rabbit polyclonal antibody to CD45 (Phospho-Y1216) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1216 of human CD45 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: PTPRC Alternative Names: CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45Entrez Gene (Human): 5788Entrez Gene (Rat) : 24699SwissProt (Human): P08575SwissProt (Mouse) : P06800SwissProt (Rat) : P04157Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD45 (Phospho-Y1216) expression in HEK293T (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates.
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Direct ELISA antibody dose-response curve using Anti-CD45 (Phospho-Y1216) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.