Description: Rabbit polyclonal antibody to iNOS (Phospho-Y151) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y151 of human iNOS protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NOS2 Alternative Names: NOS2A; Nitric oxide synthase inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2Entrez Gene (Human): 4843Entrez Gene (Mouse) : 18126Entrez Gene (Rat) : 24599SwissProt (Human): P35228SwissProt (Mouse) : P29477SwissProt (Rat) : Q06518Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of iNOS (Phospho-Y151) expression in HeLa (A), Jurkat (B), MCF7 (C) whole cell lysates.
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  Immunohistochemical analysis of iNOS (Phospho-Y151) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-iNOS (Phospho-Y151) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.