Description: Rabbit polyclonal antibody to MNK2 (Phospho-T249) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T249 of human MNK2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MKNK2 Alternative Names: GPRK7; MNK2; MAP kinase-interacting serine/threonine-protein kinase 2; MAP kinase signal-integrating kinase 2; MAPK signal-integrating kinase 2; Mnk2Entrez Gene (Human): 2872Entrez Gene (Mouse) : 17347Entrez Gene (Rat) : 299618SwissProt (Human): Q9HBH9SwissProt (Mouse) : Q8CDB0SwissProt (Rat) : Q5U2N4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of MNK2 (Phospho-T249) expression in SGC7901 (A), PC3 (B), Jurkat (C), mouse eye (D), rat brain (E) whole cell lysates.
• 
  Immunohistochemical analysis of MNK2 (Phospho-T249) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 
  Direct ELISA antibody dose-response curve using Anti-MNK2 (Phospho-T249) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.