Description: Rabbit polyclonal antibody to EPHA8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EPHA8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EPHA8 Alternative Names: EEK; HEK3; KIAA1459; Ephrin type-A receptor 8; EPH- and ELK-related kinase; EPH-like kinase 3; EK3; hEK3; Tyrosine-protein kinase receptor EEKEntrez Gene (Human): 2046Entrez Gene (Mouse) : 13842SwissProt (Human): P29322SwissProt (Mouse) : O09127SwissProt (Rat) : P29321Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EPHA8 expression in rat heart (A), mouse spleen (B), Hela (C) whole cell lysates.
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Immunohistochemical analysis of EPHA8 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.