Description: Rabbit polyclonal antibody to CDC25A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDC25A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDC25A Alternative Names: M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25AEntrez Gene (Human): 993Entrez Gene (Mouse) : 12530Entrez Gene (Rat) : 171102SwissProt (Human): P30304SwissProt (Mouse) : P48964SwissProt (Rat) : P48965Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC25A expression in CT26 (A), PC12 (B), A549 (C), LO2 (D), Hela (E) whole cell lysates.
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Immunohistochemical analysis of CDC25A staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.