Description: Rabbit polyclonal antibody to RPS15 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPS15. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RPS15 Alternative Names: RIG; 40S ribosomal protein S15; RIG proteinEntrez Gene (Human): 6209Entrez Gene (Mouse) : 20054Entrez Gene (Rat) : 29285SwissProt (Human): P62841SwissProt (Mouse) : P62843SwissProt (Rat) : P62845Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPS15 expression in SKOVCAR3 (A), HEK293T (B), A549 (C), AML12 (D), PC12 (E) whole cell lysates.
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Immunohistochemical analysis of RPS15 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.