Description: Rabbit polyclonal antibody to ITPKA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ITPKA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: ITPKA Alternative Names: Inositol-trisphosphate 3-kinase A; Inositol 145-trisphosphate 3-kinase A; IP3 3-kinase A; IP3K A; InsP 3-kinase AEntrez Gene (Human): 3706Entrez Gene (Mouse) : 228550Entrez Gene (Rat) : 81677SwissProt (Human): P23677SwissProt (Mouse) : Q8R071SwissProt (Rat) : P17105Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ITPKA expression in A549 (A), LO2 (B) whole cell lysates.
-
Immunofluorescent analysis of ITPKA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.