Description: Rabbit polyclonal antibody to GABRB2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GABRB2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GABRB2 Alternative Names: Gamma-aminobutyric acid receptor subunit beta-2; GABA(A) receptor subunit beta-2Entrez Gene (Human): 2561Entrez Gene (Mouse) : 14401Entrez Gene (Rat) : 25451SwissProt (Human): P47870SwissProt (Mouse) : P63137SwissProt (Rat) : P63138Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GABRB2 expression in C6 (A), U87MG (B), HEK293T (C), HCC827 (D), rat lung (E) whole cell lysates.
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Immunohistochemical analysis of GABRB2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.