Description: Rabbit polyclonal antibody to UCP2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UCP2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: UCP2 Alternative Names: SLC25A8; Mitochondrial uncoupling protein 2; UCP 2; Solute carrier family 25 member 8; UCPHEntrez Gene (Human): 7351Entrez Gene (Mouse) : 22228Entrez Gene (Rat) : 54315SwissProt (Human): P55851SwissProt (Mouse) : P70406SwissProt (Rat) : P56500Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of UCP2 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of UCP2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.