Description: Rabbit polyclonal antibody to IGF1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: IGF1 Alternative Names: IBP1; IGF-IA; IGF1A; IGFI; insulin-like growth factor 1; insulin-like growth factor IA; mechano growth factor; MGF; somatomedin CEntrez Gene (Human): 3479Entrez Gene (Mouse) : 16000Entrez Gene (Rat) : 24482SwissProt (Human): P05019SwissProt (Mouse) : P05017SwissProt (Rat) : P08025Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IGF1 expression in Hela (A), mouse testis (B), rat testis (C) whole cell lysates.
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Immunohistochemical analysis of IGF1 staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of IGF1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).