Description: Rabbit polyclonal antibody to IDH3 alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDH3 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: IDH3A Alternative Names: Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alphaEntrez Gene (Human): 3419Entrez Gene (Mouse) : 67834Entrez Gene (Rat) : 114096SwissProt (Human): P50213SwissProt (Mouse) : Q9D6R2SwissProt (Rat) : Q99NA5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IDH3 alpha expression in SGC7901 (A), PC12 (B), CT26 (C) whole cell lysates.
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Immunohistochemical analysis of IDH3 alpha staining in human lymph gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.