Description: Rabbit polyclonal antibody to RRP7A/B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RRP7A/B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RRP7A; RRP7B Alternative Names: RRP7A; CGI-96; Ribosomal RNA-processing protein 7 homolog A; Gastric cancer antigen Zg14; RRP7B; Putative ribosomal RNA-processing protein 7 homolog B; Putative gastric cancer antigen Zg14-like proteinEntrez Gene (Human): 27341Entrez Gene (Mouse) : 74778SwissProt (Human): Q9Y3A4; Q9NSQ0SwissProt (Mouse) : Q9D1C9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RRP7A/B expression in CT26 (A), C6 (B), HEK293T (C), A549 (D), MCF7 (E) whole cell lysates.
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Immunohistochemical analysis of RRP7A/B staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.