Description: Rabbit polyclonal antibody to Alpha-actin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ACTC1 Alternative Names: ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actinEntrez Gene (Human): 70Entrez Gene (Mouse) : 11464Entrez Gene (Rat) : 29275SwissProt (Human): P68032SwissProt (Mouse) : P68033SwissProt (Rat) : P68035Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Alpha-actin expression in C6 (A), AML12 (B) whole cell lysates.
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  Immunohistochemical analysis of Alpha-actin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.