Description: Rabbit polyclonal antibody to DCLK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DCLK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) Gene Symbol: DCLK2 Alternative Names: DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2Entrez Gene (Human): 166614Entrez Gene (Mouse) : 70762Entrez Gene (Rat) : 310698SwissProt (Human): Q8N568SwissProt (Mouse) : Q6PGN3SwissProt (Rat) : Q5MPA9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DCLK2 expression in H1792 (A), mouse testis (B), mouse heart (C) whole cell lysates.
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  Immunofluorescent analysis of DCLK2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).