Description: Rabbit polyclonal antibody to EPS8L1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPS8L1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) Gene Symbol: EPS8L1 Alternative Names: DRC3; EPS8R1; Epidermal growth factor receptor kinase substrate 8-like protein 1; EPS8-like protein 1; Epidermal growth factor receptor pathway substrate 8-related protein 1; EPS8-related protein 1Entrez Gene (Human): 54869Entrez Gene (Mouse) : 67425SwissProt (Human): Q8TE68SwissProt (Mouse) : Q8R5F8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EPS8L1 expression in HEK293T (A), H1688 (B), H446 (C), mouse kidney (D), mouse spleen (E), rat kidney (F), rat spleen (G) whole cell lysates.
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  Immunohistochemical analysis of EPS8L1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of EPS8L1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).