Description: Rabbit polyclonal antibody to CNOT7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CNOT7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CNOT7 Alternative Names: CAF1; CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1aEntrez Gene (Human): 29883Entrez Gene (Mouse) : 18983SwissProt (Human): Q9UIV1SwissProt (Mouse) : Q60809Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CNOT7 expression in HEK293T (A), mouse brain (B), rat spleen (C) whole cell lysates.
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Immunohistochemical analysis of CNOT7 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.