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Anti-CNOT7 Antibody CPA5395
  • Western blot analysis of CNOT7 expression in HEK293T (A), mouse brain (B), rat spleen (C) whole cell lysates.
  • Immunohistochemical analysis of CNOT7 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-CNOT7 Antibody
Cat No: CPA5395
Source: Rabbit
Reactivity: H, M, R, B, C, Mk
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to CNOT7
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CNOT7. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol: CNOT7
Alternative Names: CAF1; CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a
Entrez Gene (Human): 29883
Entrez Gene (Mouse) : 18983
SwissProt (Human): Q9UIV1
SwissProt (Mouse) : Q60809
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CNOT7 expression in HEK293T (A), mouse brain (B), rat spleen (C) whole cell lysates.
  • Immunohistochemical analysis of CNOT7 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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