Description: Rabbit polyclonal antibody to VPS54 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VPS54. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: VPS54 Alternative Names: HCC8; Vacuolar protein sorting-associated protein 54; Hepatocellular carcinoma protein 8; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8pEntrez Gene (Human): 51542Entrez Gene (Rat) : 286932SwissProt (Human): Q9P1Q0SwissProt (Rat) : Q9JMK8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of VPS54 expression in mouse testis (A), rat testis (B), HEK293T (C) whole cell lysates.
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Immunohistochemical analysis of VPS54 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.