Description: Rabbit polyclonal antibody to MNK1 (Phospho-T250) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T250 of human MNK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MKNK1 Alternative Names: MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1Entrez Gene (Human): 8569Entrez Gene (Mouse) : 17346Entrez Gene (Rat) : 500526SwissProt (Human): Q9BUB5SwissProt (Mouse) : O08605SwissProt (Rat) : Q4G050Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MNK1 (Phospho-T250) expression in HepG2 (A), Hela (B) whole cell lysates.
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  Immunohistochemical analysis of MNK1 (Phospho-T250) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.