Description: Rabbit polyclonal antibody to Factor XII HC Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Factor XII HC. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: F12 Alternative Names: Coagulation factor XII; Hageman factor; HAFEntrez Gene (Human): 2161Entrez Gene (Mouse) : 58992SwissProt (Human): P00748SwissProt (Mouse) : Q80YC5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Factor XII HC expression in HepG2 (A), EC9706 (B), BV2 (C) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 70; 50 kD)
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  Immunohistochemical analysis of Factor XII HC staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.