Description: Rabbit polyclonal antibody to DPD Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DPD. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DPYD Alternative Names: Dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenaseEntrez Gene (Human): 1806Entrez Gene (Mouse) : 99586Entrez Gene (Rat) : 81656SwissProt (Human): Q12882SwissProt (Mouse) : Q8CHR6SwissProt (Rat) : O89000Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DPD expression in mouse liver (A), rat liver (B) whole cell lysates.
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Immunohistochemical analysis of DPD staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.