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Anti-SPTBN1 Antibody CPA5236
  • Western blot analysis of SPTBN1 expression in Hela (A), Colo205 (B) whole cell lysates.
  • Immunohistochemical analysis of SPTBN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-SPTBN1 Antibody
Cat No: CPA5236
Source: Rabbit
Reactivity: H, M
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to SPTBN1
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPTBN1. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: SPTBN1
Alternative Names: SPTB2; Spectrin beta chain, non-erythrocytic 1; Beta-II spectrin; Fodrin beta chain; Spectrin, non-erythroid beta chain 1
Entrez Gene (Human): 6711
Entrez Gene (Mouse) : 20742
SwissProt (Human): Q01082
SwissProt (Mouse) : Q62261
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SPTBN1 expression in Hela (A), Colo205 (B) whole cell lysates.
  • Immunohistochemical analysis of SPTBN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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