Description: Rabbit polyclonal antibody to QSK Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human QSK. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SIK3 Alternative Names: KIAA0999; QSK; Serine/threonine-protein kinase SIK3; Salt-inducible kinase 3; SIK-3; Serine/threonine-protein kinase QSKEntrez Gene (Human): 23387Entrez Gene (Mouse) : 70661SwissProt (Human): Q9Y2K2SwissProt (Mouse) : Q6P4S6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of QSK expression in LO2 (A), MCF7 (B), A549 (C), CT26 (D), PC12 (E) whole cell lysates.
• 
  Immunohistochemical analysis of QSK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.