Description: Rabbit polyclonal antibody to HCN2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HCN2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HCN2 Alternative Names: BCNG2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; Brain cyclic nucleotide-gated channel 2; BCNG-2Entrez Gene (Human): 610Entrez Gene (Mouse) : 15166Entrez Gene (Rat) : 114244SwissProt (Human): Q9UL51SwissProt (Mouse) : O88703SwissProt (Rat) : Q9JKA9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HCN2 expression in SKNSH (A) whole cell lysates.
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  Immunohistochemical analysis of HCN2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.