Description: Rabbit polyclonal antibody to NUMA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NUMA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NUMA1 Alternative Names: NUMA; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigenEntrez Gene (Human): 4926SwissProt (Human): Q14980Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NUMA expression in Hela (A), H446 (B), mouse lung (C), rat lung (D), rat spleen (E) whole cell lysates.
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Immunohistochemical analysis of NUMA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.