Description: Rabbit polyclonal antibody to MCT12 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MCT12. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SLC16A12 Alternative Names: MCT12; Monocarboxylate transporter 12; MCT 12; Solute carrier family 16 member 12Entrez Gene (Human): 387700Entrez Gene (Mouse) : 240638SwissProt (Human): Q6ZSM3SwissProt (Mouse) : Q8BGC3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of MCT12 expression in HEK293T (A), A549 (B), HepG2 (C) whole cell lysates.
-
Immunohistochemical analysis of MCT12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.