Description: Rabbit polyclonal antibody to HLA-DOA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DOA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HLA-DOA Alternative Names: HLA-DNA; HLA-DZA; HLA class II histocompatibility antigen, DO alpha chain; MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOAEntrez Gene (Human): 3111SwissProt (Human): P06340Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HLA-DOA expression in K562 (A), MCF7 (B), mouse liver (C), mouse spleen (D), rat liver (E), rat spleen (F) whole cell lysates.
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Immunohistochemical analysis of HLA-DOA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.