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Anti-HLA-DOA Antibody CPA5213
  • Western blot analysis of HLA-DOA expression in K562 (A), MCF7 (B), mouse liver (C), mouse spleen (D), rat liver (E), rat spleen (F) whole cell lysates.
  • Immunohistochemical analysis of HLA-DOA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-HLA-DOA Antibody
Cat No: CPA5213
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to HLA-DOA
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DOA. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: HLA-DOA
Alternative Names: HLA-DNA; HLA-DZA; HLA class II histocompatibility antigen, DO alpha chain; MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA
Entrez Gene (Human): 3111
SwissProt (Human): P06340
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of HLA-DOA expression in K562 (A), MCF7 (B), mouse liver (C), mouse spleen (D), rat liver (E), rat spleen (F) whole cell lysates.
  • Immunohistochemical analysis of HLA-DOA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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