Description: Rabbit polyclonal antibody to POLR3A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human POLR3A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: POLR3A Alternative Names: DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160Entrez Gene (Human): 11128SwissProt (Human): O14802Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of POLR3A expression in K562 (A), MCF7 (B), COS7 (C) whole cell lysates.
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Immunohistochemical analysis of POLR3A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.