Description: Rabbit polyclonal antibody to DDX19B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DDX19B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: DDX19B Alternative Names: DBP5; DDX19; TDBP; ATP-dependent RNA helicase DDX19B; DEAD box RNA helicase DEAD5; DEAD box protein 19BEntrez Gene (Human): 11269SwissProt (Human): Q9UMR2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DDX19B expression in LO2 (A), A375 (B), mouse kindey (C) whole cell lysates.
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Immunohistochemical analysis of DDX19B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.