Description: Rabbit polyclonal antibody to ASF1B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ASF1B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ASF1B Alternative Names: Histone chaperone ASF1B; Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-IIEntrez Gene (Human): 55723SwissProt (Human): Q9NVP2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ASF1B expression in HEK293T (A), U87MG (B), Hela (C), K562 (D), MCF7 (E) whole cell lysates.
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Immunohistochemical analysis of ASF1B staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.