Description: Rabbit polyclonal antibody to SUPT16H Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SUPT16H. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SUPT16H Alternative Names: FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16Entrez Gene (Human): 11198Entrez Gene (Mouse) : 114741SwissProt (Human): Q9Y5B9SwissProt (Mouse) : Q920B9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SUPT16H expression in H446 (A), mouse lung (B), mouse kidney (C), rat lung (D) whole cell lysates.
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Immunohistochemical analysis of SUPT16H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.