Description: Rabbit polyclonal antibody to CHKB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CHKB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CHKB Alternative Names: CHETK; CHKL; Choline/ethanolamine kinase; Choline kinase beta; CK; CKB; Choline kinase-like protein; Ethanolamine kinase; EK; Ethanolamine kinase beta; EKB; choline/ethanolamine kinase beta; CKEKBEntrez Gene (Human): 1120SwissProt (Human): Q9Y259Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CHKB expression in mouse heart (A), rat heart (B) whole cell lysates.
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Immunohistochemical analysis of CHKB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.