Description: Rabbit polyclonal antibody to BCA3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: AKIP1 Alternative Names: BCA3; C11orf17; A-kinase-interacting protein 1; Breast cancer-associated gene 3 protein; PKA-interacting protein; Proline-rich protein BCA3Entrez Gene (Human): 56672Entrez Gene (Mouse) : 57373SwissProt (Human): Q9NQ31SwissProt (Mouse) : Q9JJR5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BCA3 expression in Jurkat (A), mouse spleen (B), rat spleen (C) whole cell lysates.
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Immunohistochemical analysis of BCA3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.