Description: Rabbit polyclonal antibody to LMO3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LMO3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: LMO3 Alternative Names: RBTN3; RBTNL2; RHOM3; LIM domain only protein 3; LMO-3; Neuronal-specific transcription factor DAT1; Rhombotin-3Entrez Gene (Human): 55885Entrez Gene (Mouse) : 109593SwissProt (Human): Q8TAP4SwissProt (Mouse) : Q8BZL8SwissProt (Rat) : Q99MB5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LMO3 expression in human heart (A), COS7 (B) whole cell lysates.
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Immunohistochemical analysis of LMO3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.