Description: Rabbit polyclonal antibody to BUD31 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BUD31. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: BUD31 Alternative Names: EDG2; Protein BUD31 homolog; Protein EDG-2; Protein G10 homologEntrez Gene (Human): 8896Entrez Gene (Rat) : 89819SwissProt (Human): P41223SwissProt (Rat) : O70454Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BUD31 expression in MCF7 (A), K562 (B), A549 (C), rat kidney (D) whole cell lysates.
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  Immunohistochemical analysis of BUD31 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.