Description: Rabbit polyclonal antibody to CEP135 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CEP135. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CEP135 Alternative Names: CEP4; KIAA0635; Centrosomal protein of 135 kDa; Cep135; Centrosomal protein 4Entrez Gene (Human): 9662Entrez Gene (Mouse) : 381644SwissProt (Human): Q66GS9SwissProt (Mouse) : Q6P5D4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CEP135 expression in HEK293T (A), mouse lung (B), mouse heart (C) whole cell lysates.
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Immunohistochemical analysis of CEP135 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.