Description: Rabbit polyclonal antibody to SFRS15 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SFRS15. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SCAF4 Alternative Names: KIAA1172; SFRS15; Splicing factor, arginine/serine-rich 15; CTD-binding SR-like protein RA4; SR-related and CTD-associated factor 4Entrez Gene (Human): 57466SwissProt (Human): O95104SwissProt (Rat) : Q63627Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SFRS15 expression in RT4 (A), Colo205 (B) whole cell lysates.
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Immunohistochemical analysis of SFRS15 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.