Description: Rabbit polyclonal antibody to PPM1K Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPM1K. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PPM1K Alternative Names: PP2CM; Protein phosphatase 1K mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2C-like mitochondrial protein; PP2C-type mitochondrial phosphoprotein phosphatase; PTMP; Protein phosphatase 2C isoform kappa; PP2C-kappaEntrez Gene (Human): 152926Entrez Gene (Mouse) : 243382SwissProt (Human): Q8N3J5SwissProt (Mouse) : Q8BXN7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPM1K expression in A549 (A), H1792 (B), CT26 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of PPM1K staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.