Description: Rabbit polyclonal antibody to Myosin 14 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Myosin 14. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MYH14 Alternative Names: KIAA2034; Myosin-14; Myosin heavy chain 14; Myosin heavy chain, non-muscle IIc; Non-muscle myosin heavy chain IIc; NMHC II-CEntrez Gene (Human): 79784Entrez Gene (Mouse) : 71960SwissProt (Human): Q7Z406SwissProt (Mouse) : Q6URW6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Myosin 14 expression in H1792 (A), HEK293T (B), HepG2 (C), mouse liver (D), rat liver (E) whole cell lysates.
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Immunohistochemical analysis of Myosin 14 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.