Description: Rabbit polyclonal antibody to SAR1B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAR1B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SAR1B Alternative Names: SARA2; SARB; GTP-binding protein SAR1b; GTP-binding protein B; GTBPBEntrez Gene (Human): 51128Entrez Gene (Mouse) : 66397Entrez Gene (Rat) : 287276SwissProt (Human): Q9Y6B6SwissProt (Mouse) : Q9CQC9SwissProt (Rat) : Q5HZY2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SAR1B expression in mouse brain (A), rat brain (B), HepG2 (C), HCT116 (D) whole cell lysates.
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Immunohistochemical analysis of SAR1B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.