Description: Rabbit polyclonal antibody to EIF3D Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF3D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EIF3D Alternative Names: EIF3S7; Eukaryotic translation initiation factor 3 subunit D; eIF3d; Eukaryotic translation initiation factor 3 subunit 7; eIF-3-zeta; eIF3 p66Entrez Gene (Human): 8664Entrez Gene (Mouse) : 55944Entrez Gene (Rat) : 362952SwissProt (Human): O15371SwissProt (Mouse) : O70194SwissProt (Rat) : Q6AYK8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF3D expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of EIF3D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.