Description: Rabbit polyclonal antibody to TUFM Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TUFM Alternative Names: Elongation factor Tu mitochondrial; EF-Tu; P43Entrez Gene (Human): 7284Entrez Gene (Mouse) : 233870Entrez Gene (Rat) : 293481SwissProt (Human): P49411SwissProt (Mouse) : Q8BFR5SwissProt (Rat) : P85834Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TUFM expression in Jurkat (A), Hela (B) whole cell lysates.
-
Immunohistochemical analysis of TUFM staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.