Description: Rabbit polyclonal antibody to RPL5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RPL5 Alternative Names: 60S ribosomal protein L5Entrez Gene (Human): 6125Entrez Gene (Mouse) : 100503670Entrez Gene (Rat) : 81763SwissProt (Human): P46777SwissProt (Mouse) : P47962SwissProt (Rat) : P09895Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPL5 expression in Jurkat (A), HepG2 (B) whole cell lysates.
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Immunohistochemical analysis of RPL5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.