Description: Rabbit polyclonal antibody to PHF1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PHF1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PHF1 Alternative Names: PCL1; PHD finger protein 1; Protein PHF1; hPHF1; Polycomb-like protein 1; hPCl1Entrez Gene (Human): 5252SwissProt (Human): O43189Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PHF1 expression in HeLa (A) whole cell lysates.
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Immunohistochemical analysis of PHF1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.