Description: Rabbit polyclonal antibody to CXCL1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CXCL1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CXCL1 Alternative Names: GRO; GRO1; GROA; MGSA; SCYB1; Growth-regulated alpha protein; C-X-C motif chemokine 1; GRO-alpha(1-73); Melanoma growth stimulatory activity; MGSA; Neutrophil-activating protein 3; NAP-3Entrez Gene (Human): 2919SwissProt (Human): P09341Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CXCL1 expression in HEK293T (A), H1688 (B), HCT116 (C), mouse spleen (D), mouse lung (E), rat spleen (F) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 20 kD)
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  Immunohistochemical analysis of CXCL1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.